Journal: PLOS One

Article Title: Evidence of an allostatic response by intestinal tissues following induction of joint inflammation

doi: 10.1371/journal.pone.0338053

Figure Lengend Snippet: (A) At time of sacrifice, the diameters of knee joints were measured as described in the Method from rats without treatment (Pre-Treat), after IAI of PBS and 3 days (3d), 7 days (7d) and 14 days (14d) post-CFA. CFA-IAI knees showed significant swelling at all three time points, when compared to pre-treatment or PBS-IAI (*** = p < 0.001). (B,C) Abundance of bikunin species in synovial fluids ( B ) and sera ( C ) were determined by western blotting as described in the Methods and Supporting Information . Note that for pre-treatment (Pre-Treat) and PBS groups, the measurements at 0d and 14d, respectively, were combined since no significant differences were observed between those two groups (general linear model 0d v. 3d v. 14d PBS serum p = 0.226). For A-C * = p < 0.05, ** = p < 0.01, *** = p < 0.001. Coronal formalin fixed paraffin embedded (FFPE) sections of pretreatment and 14d IAI-CFA knee joints were stained with H&E ( D & G ), anti-CD68 ( E & H ) or anti-CD4 ( F & I ). The CFA-induced synovial hyperplasia enriched in collagenous ECM and infiltrated by CD68 + macrophages is indicated by black arrows and the swollen patellar tendon by (*). M = meniscus, Fc = femoral condyles, TP = tibial plateau, S = synovium, PT = patellar tendon.

Article Snippet: Sections of intestinal Swiss rolls were stained using the Alcian Blue Kit (Vector Labs, H-3501), anti-CD68 (1:100), anti-CD4 (1:2000), anti-CD8 (Abcam, Ab237709 , 1:500) or anti-Ki67 (Invitrogen, MA5–14520; 1:100), Invitrogen, MA5–14520) or the following biotinylated lectins (all from Vector Labs), Griffonia Bandeiraea Simplidifolia Lectin I, (GSL I, B-1105–2), Maackia Amurensis Lectin II (MAL II, B-1265–1) and Ulex Europaeus Agglutinin I (UEA, B-1065–2).

Techniques: Western Blot, Formalin-fixed Paraffin-Embedded, Staining

Journal: PLOS One

Article Title: Evidence of an allostatic response by intestinal tissues following induction of joint inflammation

doi: 10.1371/journal.pone.0338053

Figure Lengend Snippet: Adjacent FFPE-sections from proximal and distal regions of the ileum and colon were stained with ( A ) anti-CD68 to localize macrophages or ( B ) anti-CD4 and ( C ) anti-CD8 to localize T-cells. Black arrows indicate regions of increased reactivity post CFA. ( D ) DEGs typically associated with intestinal macrophages or T cells caused by CFA-IAI are summarized in the heat map. PI = Proximal Ileum, DI = Distal Ileum, PC = Proximal Colon, DC = Distal Colon.

Article Snippet: Sections of intestinal Swiss rolls were stained using the Alcian Blue Kit (Vector Labs, H-3501), anti-CD68 (1:100), anti-CD4 (1:2000), anti-CD8 (Abcam, Ab237709 , 1:500) or anti-Ki67 (Invitrogen, MA5–14520; 1:100), Invitrogen, MA5–14520) or the following biotinylated lectins (all from Vector Labs), Griffonia Bandeiraea Simplidifolia Lectin I, (GSL I, B-1105–2), Maackia Amurensis Lectin II (MAL II, B-1265–1) and Ulex Europaeus Agglutinin I (UEA, B-1065–2).

Techniques: Staining